Journal: Contrast Media & Molecular Imaging

Article Title: High Contrast PET Imaging of GRPR Expression in Prostate Cancer Using Cobalt-Labeled Bombesin Antagonist RM26

doi: 10.1155/2017/6873684

Figure Lengend Snippet: (a) In vitro specificity test of 57 Co-NOTA-PEG 2 -RM26 binding to GRPR on PC-3 (human prostate cancer) cells. Presaturation of receptors with unlabeled NOTA-PEG 2 -RM26 caused significant ( p < 0.05) reduction of cell-bound 57 Co-NOTA-PEG 2 -RM26 radioactivity. (b) Binding and internalization of 57 Co-NOTA-PEG 2 -RM26 at 37°C by PC-3 cells. Data are normalized to a maximum cell-bound radioactivity and presented as average value from 3 cell dishes ± SD. Not all error bars are visible due to the small standard deviations.

Article Snippet: PC-3 human prostate cancer cells expressing GRPR were purchased form ATCC via LGC Promochem.

Techniques: In Vitro, Binding Assay, Radioactivity

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Abyssinone V-4' Methyl Ether Isolated from Erythrina droogmansiana (Leguminosae) Inhibits Cell Growth and Mammary Glands Hyperplasia Induced in Swiss Mice by the 7,12-Dimethylbenz(a)anthracene

doi: 10.1155/2018/7959068

Figure Lengend Snippet: Growth of DU145, PC3, HepG2, and MCF-7 carcinoma cells treated with different concentrations of Abyssinone V-4' methyl ether (AVME) after 24, 48, and 72 hours. Controls remained untreated (n = 3).

Article Snippet: The following cancer cell lines, MCF-7 (human ER-positive breast adenocarcinoma cells), HepG2 (human liver hepatocellular carcinoma), and DU145 and PC3 (human androgens independent prostate carcinoma) cells, were acquired from LGC Promochem (Wesel, Germany).

Techniques:

Journal: Scientific Reports

Article Title: Optimization of HER3 expression imaging using affibody molecules: Influence of chelator for labeling with indium-111

doi: 10.1038/s41598-018-36827-w

Figure Lengend Snippet: Binding specificity of 111 In-Z 08698 -X on BxPC-3 and DU145 cells. HER3 receptors in the blocked group were pre-saturated with excess of unlabeled Z 08698 . Data are presented as an average ± standard deviation from three cell culture dishes.

Article Snippet: HER3 expressing cell lines, BxPC-3 (pancreatic cancer) and DU145 (prostate cancer), were purchased from American Type Tissue Culture Collection (ATTC via LGC Promochem, Borås, Sweden) and cultured in RMPI-1640 media supplemented with 1% penicillin and 1% L-glutamine (all from Biochrom, Berlin, Germany) and 10% fetal bovine serum (Sigma-Aldrich, Germany) at 37 °C with 5% CO 2 .

Techniques: Binding Assay, Standard Deviation, Cell Culture

Journal: Scientific Reports

Article Title: Optimization of HER3 expression imaging using affibody molecules: Influence of chelator for labeling with indium-111

doi: 10.1038/s41598-018-36827-w

Figure Lengend Snippet: Cellular processing of 111 In-Z 08698 -X on BxPC-3 (black) and DU145 cells (grey). Total cell bound activity (solid line) and internalized activity (dashed line) are displayed as percent of cell associated activity. The values were normalized to maximum cell-associated activity for each conjugate. Data are presented as an average ± standard deviation from three cell culture dishes.

Article Snippet: HER3 expressing cell lines, BxPC-3 (pancreatic cancer) and DU145 (prostate cancer), were purchased from American Type Tissue Culture Collection (ATTC via LGC Promochem, Borås, Sweden) and cultured in RMPI-1640 media supplemented with 1% penicillin and 1% L-glutamine (all from Biochrom, Berlin, Germany) and 10% fetal bovine serum (Sigma-Aldrich, Germany) at 37 °C with 5% CO 2 .

Techniques: Activity Assay, Standard Deviation, Cell Culture